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Treat cells by adding fresh media containing regulator for desired time. Drain membrane of excess developing solution.

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Electrotransfer to nitrocellulose membrane Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature. Do not let dry. Primary Antibody Dilution Buffer: Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: